Eric C. Greene, PhD

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Overview

Academic Appointments

  • Professor of Biochemistry and Molecular Biophysics

Eric Greene is a professor at Columbia University, New York, New York, USA, in the Department of Biochemistry and Molecular Biophysics. He received his Ph.D. in biochemistry from Texas A&M University, College Station, USA, while working for Dorothy Shippen and conducted postdoctoral research with Kiyoshi Mizuuchi at the National Institutes of Health, Bethesda, Maryland, USA. His laboratory uses novel approaches to single-molecule imaging for studying problems related to genome integrity. He enjoys fly fishing. 

Research

Our group uses single-molecule optical microscopy to study fundamental interactions between proteins and nucleic acids. Our overall goal is to reveal the molecular mechanisms that cells use to repair, maintain, and decode their genetic information. This research combines aspects of biochemistry, physics, and nanoscale technology to answer questions about complex biological problems that cannot be easily addressed through traditional biochemical approaches. As part of our work, we have established robust experimental platforms that enable single molecule imaging of biochemical reaction mechanisms in a “high throughput” experimental format that can be applied to the study of protein-nucleic acid interactions. The advantages of our approaches are that we can see what proteins are bound to DNA, where they are bound, how they move, and how they interact with and influence other components of the system – all in real-time, at the level of a single reaction. We are applying this technology towards determining the physical basis for the mechanisms that proteins use to maintain genome integrity, with particular emphasis on reactions related to homologous DNA recombination. 

Grants

DEFINING THE CONTRIBUTIONS OF BRCA1, BRCA2, AND RAD52 TO GENOME STABILITY (Federal Gov)

Feb 11 2020 - Jan 31 2025

HELICASE REGULATION DURING HOMOLOGOUS RECOMBINATION (Federal Gov)

Mar 1 2019 - Feb 29 2024

MECHANICS AND EXECUTION OF HOMOLOGOUS RECOMBINATION - BIOPHYSICS TO THE ORGANISM (Private)

Jan 1 2018 - Dec 31 2022

EVOLUTIONARY ORIGINS AND IMPLICATIONS OF RAD51 AND DMC1 (Federal Gov)

Jul 1 2018 - Jun 30 2022

STRUCTURAL CELL BIOLOGY OF DNA REPAIR MACHINES (Federal Gov)

Sep 13 2016 - Aug 31 2021

VISUALIZING THE STRAND INVASION DURING HOMOLOGOUS RECOMBINATION ON THE SINGLE MOLECULE LEVEL (Private)

Jul 1 2017 - Jun 30 2021

MECHANISMS OF GENOME INTEGRITY (Federal Gov)

May 1 2016 - Apr 30 2021

UNDERSTANDING THE BASIS OF FIDELITY IN EUKARYOTIC RECOMBINASES (Federal Gov)

Sep 1 2016 - Aug 31 2019

PEW LATIN AMERICAN FELLOWS PROGRAM (Private)

Aug 1 2014 - Jul 31 2017

USING DNA CURTAINS TO REVEAL THE MECHANISMS OF TARGET SITE L OCATION BY DNA BINDING PROTEINS (Federal Gov)

Jun 1 2012 - May 31 2017

VISUALIZING THE DYNAMICS OF CHROMATIN AND CHROMATIN REMODELING PROTEINS (Federal Gov)

Jul 15 2008 - Nov 30 2016

ELUCIDATING THE MECHANISMS OF DNA RECOMBINATION (Federal Gov)

Aug 1 2012 - Apr 30 2016

USING NNOFABRICATED DNA CURTAINS TO REVEAL PROTEIN DURING REPLICATION (Federal Gov)

Sep 1 2012 - Aug 31 2014

MECHANISMS OF DNA MOTOR PROTEINS IN GENOME MAINTENANCE (Federal Gov)

Aug 1 2009 - May 31 2014

CAREER: USING HIGH-THROUGHPUT SINGLE-MOLECULE ANALYSIS TO RE VEAL THE MECHANISMS OF TARGET SITE LOCATION BY DNA REPAIR PR (Federal Gov)

May 15 2006 - Apr 30 2012

INTRON ENDONUCLEASESE AND INTEINS (Federal Gov)

Aug 3 2009 - Jun 30 2011

COORDINATION OF BRCA1/BARD1-AND MRE11/RAD50/NBS1-DEPENDENT D NA TRANSACTIONS IN BREAST TUMOR SUPPRESS (Federal Gov)

Jul 1 2008 - Jun 30 2011

SINGLE MOLECULE STUDIES OF DNA ERROR RECOGNITION BY MISMATCH (Federal Gov)

Jun 1 2007 - May 31 2010

ELUCIDATING THE MECHANISMS OF DNA DAMAGE RECOGNITION THROUGH THE DIRECT OBSERVATION OF SINGLE REACTIONS (Private)

Jan 1 2005 - Dec 31 2009

MECHANISM OF HOMOLOGY SEARCH IN HOMOLOGOUS RECOMBINATION BY RAD51 AND RAD54 (Federal Gov)

Apr 1 2008 - Sep 30 2009

IMAGING THE MOLECULAR MECHANISMS OF DEOXYRIBONUCLEIC ACID DA MAGE REPAIR (Private)

May 1 2005 - Apr 30 2009